ZymeLeap™

Engineered to screen faster.
Designed to power your pipeline.

Accelerating Enzyme Engineering Beyond Legacy Methods

ZymeLeap™ is a proprietary platform purpose-built for scalable enzyme engineering. Integrating ultra-high-throughput microfluidics screening, computational design, and machine learning, we screen millions of enzyme variants beyond the limits of legacy methods.

01
Ultra-high-throughput droplet screening

Large enzyme libraries containing millions of variants are compartmentalized into picoliter-sized droplets using our proprietary microfluidics platform. Generated at frequencies of 5–6 kHz, each droplet functions as an individual microreactor for quantitative activity screening. Integrated fluorescence detection and high-throughput sorting enable the screening of over 10⁷ enzyme variants per day while minimizing reagent consumption and expanding accessible sequence space.

02
ML-guided design powered by an evolving enzyme knowledge database

An AI-driven computational enzyme design function that integrates sequence, structural, modelling, and experimental screening data into actionable engineering workflows. From sequence discovery and hotspot selection to hit prioritization and combinatorial library design, it enables exploration of enzyme sequence space beyond the reach of conventional approaches.

03
High flexibility library design with precision enzyme engineering

Flexible library design strategies enable targeted exploration of enzyme sequence space across diverse performance objectives. By integrating screening data on activity, stability, and specificity into iterative engineering workflows, the platform continuously refines variant selection and accelerates the development of application-ready biocatalysts.

A Workflow Engineered
for Performance

A closed-loop engineering workflow designed to continuously learn, refine, and improve enzyme performance across every stage of discovery and development.

Platform
ZymeLeap
Design · Build · Test · Learn
D
In-silico Design
Top enzyme from discovery workflow · Libraries built using different strategies
We combine computational modelling, sequence analysis, and rational library design to generate high-quality enzyme variants tailored to specific performance goals. By integrating data from previous screening campaigns, we design focused libraries that improve hit rates while reducing experimental complexity.
B
Library Build
In-house library build
We use molecular biology tools to construct high-density libraries.
  • Single-site saturation mutagenesis
  • Multiple-site saturation mutagenesis
  • Homologous recombination mutagenesis
  • DNA shuffling
  • epPCR
T
Ultra-high-throughput Screening
Core: Microfluidics
Allozymes’ microfluidic technology miniaturizes and automates enzymatic reactions, enabling custom assay development and rapid screening of large enzyme libraries under diverse conditions.
  • Precise proprietary enzymatic assay with minimum noise
  • Multifaceted screenings for each selection pressure or combination of them
  • Affordable screenings with cost-prohibitive substrates
L
Data Analysis
Comprehensive sequence-activity datasets
Every droplet becomes a data point, generating comprehensive sequence-activity datasets that power machine learning-guided enzyme engineering and smarter library design.
  • Machine learning models trained on proprietary datasets
  • Prioritisation of high-performing variants and hits
  • Accelerated optimisation cycles through data-driven iteration
  • Deep sequencing and analytical validation of top variants
In-house Enzyme Production
Upstream and downstream capabilities
INTEGRATED OPERATIONAL SUPPORT CAPABILITIES

Built for Industrial Readiness

Built to support real-world development, our in-house upstream and downstream capabilities enable enzyme production, process validation, and pilot-scale readiness under conditions designed for industrial manufacturing environments.

Upstream
Upstream Process Development
Small-Scale Validation
1.5 L & 14 L Fermenters

Tested in controlled bioreactor conditions to reflect real process environments.

Small-scale bioreactor systems used for upstream enzyme process development at Allozymes
Pilot-Scale Readiness
150 L Piloting

Ensuring enzyme performance translates reliably from lab to pilot-scale conditions.

Pilot-scale fermentation equipment supporting enzyme scale-up and process translation at Allozymes
Small-scale bioreactor systems used for upstream enzyme process development at Allozymes
Pilot-scale fermentation equipment supporting enzyme scale-up and process translation at Allozymes
Downstream
Downstream Integration

Cell lysis, separation, chromatography, and drying workflows to support integrated enzyme production.

Downstream processing laboratory supporting enzyme purification and integrated biomanufacturing workflows at Allozymes
Downstream processing laboratory supporting enzyme purification and integrated biomanufacturing workflows at Allozymes
Enzyme Supply
Quality-Controlled Enzyme Supply

QA/QC processes support enzyme quality, consistency, and release testing, with CoA documentation provided for supplied enzymes.

Designed to support regulatory testing, process transfer, and early commercial launch preparation.

Quality-controlled enzyme supply and packaging for industrial biocatalysis applications at Allozymes
Quality-controlled enzyme supply and packaging for industrial biocatalysis applications at Allozymes

Where scalable biology begins